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Issue: Issue 3 (2016) – Supplement 1


Electroacupuncture pretreatment at GB20 exerts anti-nociceptive effects via peripheral and central serotonin mechanism in conscious migraine rats

Lu Liu, P. Pei, L. Zhao, Z. Qu, and L. Wang
Background: While electroacupuncture (EA) pretreatment in migraine has been found to attenuate pain and frequencies of attacks, the mechanism of its anti-nociceptive effect remain poorly understood. Emerging evidence suggests that peripheral and central serotonin system may be involved in migraine. We hypothesized that EA would exert anti-nociceptive effects via modulation of serotonin system. Methods: We used a conscious migraine model induced by repeated electrical stimulation on the dural mater. Forty rats were randomly assigned to one of four groups: an EA group, which received EA at GB20 following dural stimulation; a sham acupuncture group, which received manual acupuncture at a non-acupuncture point following dural stimulation; a Model group, which received dural stimulation but no acupuncture; and a Control group, which received neither dural stimulation nor acupuncture. HomeCageScan was used to measure effects on the spontaneous nociceptive behaviors, radioimmunoassay and HPLC were used to investigate the expression of 5-HT in peripheral plasma and in the periaqueductal gray (PAG), rostroventromedial medulla (RVM), and trigeminal nucleus caudalis (TNC), three key structure of the descending pain modulatory system. Results: Our study showed that EA pretreatment could produce a significant reduction in resting, freezing and grooming, and a significant increase in exploration behavior. We found that the level of 5-HT in plasma were significantly increased, while significantly reduced in RVM, TNC in Model group. Furthermore, the above results were significantly reversed in EA at GB20. Conclusions: EA pretreatment exerts anti-nociceptive effects in a rat model of recurrent migraine, possibly via modulation of the serotonin system.

Special Issue on Controversies in Neurology. From the 10th World Congress on Controversies in Neurology (CONy), Lisbon, Portugal. 17–20 March 2016.

International Journal of Clinical Neurosciences and Mental Health 2016; 3(Suppl. 1):P66
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